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A carbohydrate-based mechanism of species recognition in sea urchin fertilization BJMBR
Mourão,P.A.S..
In the present review, we describe a systematic study of the sulfated polysaccharides from marine invertebrates, which led to the discovery of a carbohydrate-based mechanism of sperm-egg recognition during sea urchin fertilization. We have described unique polymers present in these organisms, especially sulfated fucose-rich compounds found in the egg jelly coat of sea urchins. The polysaccharides have simple, linear structures consisting of repeating units of oligosaccharides. They differ among the various species of sea urchins in specific patterns of sulfation and/or position of the glycosidic linkage within their repeating units. These polysaccharides show species specificity in inducing the acrosome reaction in sea urchin sperm, providing a clear-cut...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acrosome reaction; Fertilization; Sulfated fucans; Glycosaminoglycans; Sulfated galactans; Species diversity; Sperm-egg recognition.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100002
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Induction of the acrosome reaction test to in vitro estimate embryo production in Nelore cattle Arq. Bras. Med. Vet. Zootec.
Costa,M.Z.; Oliveira,L.Z.; Resende,M.V.; Lucio,A.C.; Perini,A.P.; Miguel,M.C.V.; Lima,V.F.M.H..
The effectiveness of induction of the acrosome reaction (AR) test as a parameter to in vitro estimate embryo production (IVP) in Nelore breed and the AR pattern by the Trypan Blue/Giemsa (TB) stain were evaluated. Frozen semen samples from ten Nelore bulls were submitted to AR induction and were also evaluated for cleavage and blastocyst rates. The treatments utilized for AR induction were: control (TALP medium), TH (TALP medium + 10μg heparin), TL (TALP medium + 100μg lysophosphatidylcholine) and THL (TALP medium + 10μg heparin + 100μg lysophosphatidylcholine). Sperm acrosomal status and viability were evaluated by TB staining at 0 and after 4h incubation at 38°C. The results obtained for AR presented a significant difference (P<0.05) in the percentage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Fertility; Semen; Acrosome reaction; Fertilization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400001
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Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neoglycoprotein BJMBR
Passos,E.P.; Brugnara,L.; Facin,A.C.; Riffel,A.; Lima,G.R.; Freitas,V.; Brandelli,A..
The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acrosome reaction; Endometriosis; Infertility; Peritoneal fluid; Sperm.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100009
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